Expression and purification of a biologically active Phytophthora parasitica cellulose binding elicitor lectin in Pichia pastoris
01/12/2011 00:00
Mathieu Larroque, Diana Ramirez, Claude Lafitte, Gisèle Borderies, Bernard Dumas and Elodie Gaulin
Résumé:
The Phytophthora parasitica cellulose-binding elicitor lectin, (CBEL), is a cell wall-localized protein playing a key role in cell wall organization and adhesion of the mycelium to cellulosic substrates. CBEL is a potent elicitor of plant immune responses and this activity is linked to its ability to bind plant cell wall components. In order to scale up the production of active...