Liste des publications scientifiques

Etude de composés à intérêts technologique et fonctionnel dans la bière

28/03/2011 00:00
Céline Leitao   Résumé: L’objectif de ce travail était de développer une boisson maltée originale qui se différencierait des produits existants sur le marché par ses propriétés organoleptiques, notamment par son aspect incolore ou l’ajout de couleurs vives et par la mise en évidence de ses propriétés nutritionnelles et fonctionnelles. Une décoloration avec du charbon actif en poudre a permis d’obtenir une bière décolorée qui pourra aboutir à un produit aux couleurs originales. Le travail...

Effects of processing steps on the phenolic content and antioxidant activity of beer

21/01/2011 00:00
Leitao C, Marchioni E, Bergaentzlé M, Zhao M, Didierjean L, Taidi B, Ennahar S.   Résumé: A new analytical method (liquid chromatography−antioxidant, LC-AOx) was used that is intended to separate beer polyphenols and to determine the potential antioxidant activity of these constituents after they were allowed to react online with a buffered solution of the radical cation 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+). Using the LC-AOx method, it was possible to demonstrate...

Phospholipases in action during plant defense signaling

13/01/2011 00:00
Joanne Canonne, Solène Froidure-nicolas and Susana Rivas   Résumé: Eukaryotic organisms rely on intricate signaling networks to connect recognition of microbes with the activation of efficient defense reactions. Accumulating evidence indicates that phospholipids are more than mere structural components of biological membranes. Indeed, phospholipid-based signal transduction is widely used in plant cells to relay perception of extracellular signals. Upon perception of the invading microbe,...

Dihydrosphingosine-Induced Programmed Cell Death in Tobacco BY-2 Cells Is Independent of H2O2 Production

03/01/2011 00:00
Christophe Lachaud, Daniel Da Silva, Nicolas Amelot, Chloé Béziat, Christian Brière, Valérie Cotelle, Annick Graziana, Sabine Grat, Christian Mazars and Patrice Thuleau   Résumé: Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid Long Chain Base (LCB) in plants, has been recently shown to induce both cytosolic and nuclear calcium transient increases and a correlated Programmed Cell Death (PCD) in tobacco BY-2 cells. In this study, in order to get deeper...

Detection and functional characterization of a 215 amino acid N-terminal extension in the Xanthomonas type III effector XopD

22/12/2010 00:00
Joanne Canonne, Daniel Marino, Laurent D. Noël, Ignacio Arechaga, Carole Pichereaux, Michel Rossignol, Dominique Roby and Susana Rivas   Résumé: During evolution, pathogens have developed a variety of strategies to suppress plant-triggered immunity and promote successful infection. In Gram-negative phytopathogenic bacteria, the so-called type III protein secretion system works as a molecular syringe to inject type III effectors (T3Es) into plant cells. The XopD T3E from the strain 85-10 of...

Predicting the substrate specificity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit

17/12/2010 00:00
Thomas Louveau, Céline Leitao, Sol Green, Cyril Hamiaux, Benoît van der Rest, Odile Dechy-Cabaret, Ross G. Atkinson and Christian Chervin   Résumé: The volatile compounds that constitute the fruit aroma of ripe tomato (Solanum lycopersicum) are often sequestered in glycosylated form. A homology-based screen was used to identify the gene SlUGT5, which is a member of UDP-glycosyltransferase 72 family and shows specificity towards a range of substrates, including flavonoid,...

Both Plant and Bacterial Nitrate Reductases Contribute to Nitric Oxide Production in Medicago truncatula Nitrogen-Fixing Nodules

07/12/2010 00:00
Faouzi Horchani, Marianne Prévot, Alexandre Boscari, Edouard Evangelisti, Eliane Meilhoc, Claude Bruand, Philippe Raymond, Eric Boncompagni, Samira Aschi-Smiti, Alain Puppo and Renaud Brouquisse   Résumé: Nitric oxide (NO) is a signaling and defense molecule of major importance in living organisms. In the model legume Medicago truncatula, NO production has been detected in the nitrogen fixation zone of the nodule, but the systems responsible for its synthesis are yet unknown and its role...

Autoacetylation of the Ralstonia solanacearum effector PopP2 targets a lysine residue essential for RRS1-R-mediated immunity in Arabidopsis

18/11/2010 00:00
Tasset C, Bernoux M, Jauneau A, Pouzet C, Brière C, Kieffer-Jacquinod S, Rivas S, Marco Y, Deslandes L   Résumé: Type III effector proteins from bacterial pathogens manipulate components of host immunity to suppress defence responses and promote pathogen development. In plants, host proteins targeted by some effectors called avirulence proteins are surveyed by plant disease resistance proteins referred to as “guards”. The Ralstonia solanacearum effector protein PopP2 triggers immunity in...

The regulation of arbuscular mycorrhizal symbiosis by phosphate in pea involves early and systemic signalling events

02/11/2010 00:00
Coline Balzergue, Virginie Puech-Pagès, Guillaume Bécard and Soizic F. Rochange   Résumé: Most plants form root symbioses with arbuscular mycorrhizal (AM) fungi, which provide them with phosphate and other nutrients. High soil phosphate levels are known to affect AM symbiosis negatively, but the underlying mechanisms are not understood. This report describes experimental conditions which triggered a novel mycorrhizal phenotype under high phosphate supply: the interaction between pea and...

Insights into the extracytoplasmic stress response of Xanthomonas campestris pv. campestris: role and regulation of {sigma}E-dependent activity

22/10/2010 00:00
Bordes P, Lavatine L, Phok K, Barriot R, Boulanger A, Castanié-Cornet MP, Déjean G, Lauber E, Becker A, Arlat M, Gutierrez C.   Résumé: Xanthomonas campestris pv. campestris is an epiphytic bacterium that can become a vascular pathogen responsible for black rot disease of crucifers. To adapt gene expression in response to ever-changing habitats, phytopathogenic bacteria have evolved signal transduction regulatory pathways, such as extracytoplasmic function (ECF) σ factors. The alternative...
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