Liste des publications scientifiques

Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates

09/07/2015 17:18
Emilie Widemann, Bernard Grausem, Hugues Renault, Emmanuelle Pineau, Clément Heinrich, Raphaël Lugan, Pascaline Ullmann, Laurence Miesch, Yann Aubert, Michel Miesch, Thierry Heitz and Franck Pinot   Résumé: The role and fate of Jasmonoyl-Phenylalanine (JA-Phe), an understudied conjugate in the jasmonate pathway remain to be unraveled. We addressed here the possibility of JA-Phe oxidative turnover by cytochrome P450s of the CYP94 family. Leaf wounding or fungal infection in Arabidopsis...

Bacillus subtilis SalA is a phosphorylation-dependent transcription regulator which represses scoC and activates the production of the exoprotease AprE

19/06/2015 11:50
Abderahmane Derouiche, Lei Shi, Vladimir Bidnenko, Magali Ventroux, Nathalie Pigonneau, Mirita Franz-Wachtel, Aida Kalantari, Sylvie Nessler, Marie-Françoise Noirot-Gros and Ivan Mijakovic   Résumé: Bacillus subtilis Mrp family protein SalA has been shown to indirectly promote the production of the exoprotease AprE by inhibiting the expression of scoC, which codes for a repressor of aprE. The exact mechanism by which SalA influences scoC expression has not been clarified previously. We...

Dynamic changes in ANGUSTIFOLIA3 complex composition reveal a growth regulatory mechanism in the maize leaf

08/06/2015 11:47
Hilde Nelissen, Dominique Eeckhout, Kirin Demuynck, Geert Persiau, Alan Walton, Michiel van Bel, Marieke Vervoort, Jasper Candaele, Jolien De Block, Stijn Aesaert, Mieke Van Lijsebettens, Sofie Goormachtig, Klaas Vandepoele, Jelle Van Leene, Michael Muszynski, Kris Gevaert, Dirk Inzéa and Geert De Jaeger   Résumé: Most molecular processes during plant development occur with a particular spatio-temporal specificity. Thus far, it has remained technically challenging to capture dynamic...

Use of Plasmodium falciparum culture-adapted field isolates for in vitro exflagellation-blocking assay

04/06/2015 11:43
Louis-Jérôme Leba, Lise Musset, Stéphane Pelleau, Yannick Estevez, Caroline Birer, Sébastien Briolant, Benoit Witkowski, Didier Ménard, Michael J Delves, Eric Legrand, Christophe Duplais and Jean Popovici   Résumé: Background A major requirement for malaria elimination is the development of transmission-blocking interventions. In vitro transmission-blocking bioassays currently mostly rely on the use of very few Plasmodium falciparum reference laboratory strains isolated decades ago. To...

A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity

21/05/2015 00:00
Clémentine Le Roux, Gaëlle Huet, Alain Jauneau, Laurent Camborde, Dominique Trémousaygue, Alexandra Kraut, Binbin Zhou, Marie Levaillant, Hiroaki Adachi, Hirofumi Yoshioka, Sylvain Raffaele, Richard Berthomé, Yohann Couté, Jane E. Parker and Laurent Deslandes   Résumé: Microbial pathogens infect host cells by delivering virulence factors (effectors) that interfere with defenses. In plants, intracellular nucleotide-binding/leucine-rich repeat receptors (NLRs) detect specific effector...

A petunia GRAS transcription factor controls symbiotic gene expression and fungal morphogenesis in arbuscular mycorrhiza

12/05/2015 00:00
Mélanie, K Rich, Martine Schorderet, Laure Bapaume, Laurent Falquet, Patrice Morel, Michiel Vandenbussche and Didier Reinhardt   Résumé: Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves a complex symbiotic interface over which nutrients are exchanged between the plant host and the AM fungus. Dozens of genes in the host are required for the establishment and functioning of the interaction, among them nutrient transporters that mediate the uptake of mineral nutrients delivered...

Microbial invertases: A review on kinetics, thermodynamics, physiochemical properties

05/05/2015 00:00
Habibullah Nadeem, Muhammad Hamid Rashid, Muhammad Hussnain Siddique, Farrukh Azeema, Saima Muzammil, Muhammad Rizwan Javed, Muhammad Amjad Ali, Ijaz Rasul, Muhammad Riaz   Résumé: Invertase (EC.3.2.1.26) belongs to glycoside hydrolases that catalyzes sucrose (table sugar) into two monosaccharides i.e. glucose and fructose. Invertases produced by a large number of microorganisms having molecular mass in the range of 47–430 kDa, Km value from 0.063 to 470 mM, Kcat from 5.68 to...

Transcriptome analysis highlights preformed defenses and signaling pathways controlled by the prAe1 QTL, conferring partial resistance to Aphanomyces euteiches in Medicago truncatula

04/05/2015 00:00
Yacine Badis, Maxime Bonhomme, Claude Lafitte, Stéphanie Huguet, Sandrine Balzergue, Bernard Dumas and Christophe Jacquet   Résumé: To gain an insight into the molecular mechanisms of quantitative disease resistance in Medicago truncatula to the root-infecting oomycete Aphanomyces euteiches, we selected two near-isogenic lines (NILs), NR and NS, partially resistant and susceptible, respectively, differing in the allelic state of the quantitative resistance locus (QRL) prAe1 (partially...

Imaging and analysis of mitochondrial dynamics in living cells

30/04/2015 00:00
Sanjaya B. Ekanayake, Amr M. El Zawily, Gaël Paszkiewicz, Aurélia Rolland, David C. Logan   Résumé: One of the most striking features of plant mitochondria when visualized in living tissue is their dynamism. The beauty of cytoplasmic streaming, driving, and being driven by the motility of mitochondria and other small organelles belies the complexity of the process. Equally, capturing that dynamism and investigating the genes, proteins, and mechanisms underpinning the processes using...

CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection

22/04/2015 00:00
Yann Aubert, Emilie Widemann, Laurence Miesch, Franck Pinot and Thierry Heitz   Résumé: Induced resistance to the necrotrophic pathogen Botrytis cinerea depends on jasmonate metabolism and signalling in Arabidopsis. We have presented here extensive jasmonate profiling in this pathosystem and investigated the impact of the recently reported jasmonoyl-isoleucine (JA-Ile) catabolic pathway mediated by cytochrome P450 (CYP94) enzymes. Using a series of mutant and overexpressing (OE) plant...
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