Eleven microsatellite markers for the mountain clover Trifolium montanum (Fabaceae)

Matter P, Määttänen K, Kettle CJ, Ghazoul J, Pluess AR

Résumé:

Microsatellite markers were developed in Trifolium montanum to investigate pollen dispersal patterns at landscape scale with a pollen-pool analysis (indirect parentage analysis) as well as in an experimental set-up with a direct paternity analysis.

Screening 46 microsatellites developed for T. repens yielded four markers usable in T. montanum. Seven additional ones have been developed specifically for the target species using a 454-sequencing approach. All markers were polymorphic, with an allele number ranging from two to 45 based upon 254 individuals sampled from four populations, and an exclusion probability of 0.999.

These markers proved a useful and reliable molecular tool for use in population genetics and parentage studies of this common grassland herb.

Revue:

American Journal of Botany

Lien:

www.amjbot.org/content/99/11/e447.long