Liste des publications scientifiques

Etude fonctionelle des enzymes RNaseIII chez les plantes

23/09/2013 00:00
Nahid Shamandi   Résumé: Chez la majorité des eucaryotes, les petits ARN (miRNA et siRNA) jouent des rôles essentiels au cours du développement, dans les réponses adaptatives aux stress, et dans la maintenance de la stabilité génétique. Les plantes codent quatre enzymes RNaseIII de type DICER-LIKE (DCL). DCL1, produit les miRNAs, tandis que DCL2, DCL3 et DCL4 produisent des siRNAs des tailles diverses. Les plantes codent également des enzymes appelées RNASE-THREE-LIKE (RTL) auxquelles il...

The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves

19/09/2013 00:00
Widemann E, Miesch L, Lugan R, Holder E, Heinrich C, Aubert Y, Miesch M, Pinot F, Heitz T.   Résumé: Jasmonates (JAs) are a class of signaling compounds that mediate complex developmental and adaptative responses in plants. JAs derive from jasmonic acid (JA) through various enzymatic modifications, including conjugation to amino acids or oxidation, yielding an array of derivatives. The main hormonal signal, jasmonoyl-l-isoleucine (JA-Ile), has been found recently to undergo catabolic...

Predicting promoters targeted by TAL effectors in plant genomes: from dream to reality

03/09/2013 00:00
Laurent D Noël, Nicolas Denancé, Boris Szurek   Résumé: Transcription Activator-Like (TAL) effectors from the plant pathogenic bacteria of the genus Xanthomonas are molecular weapons injected into eukaryotic cells to modulate the host transcriptome. Upon delivery, TAL effectors localize into the host cell nucleus and bind to the promoter of plant susceptibility (S) genes to activate their expression and thereby facilitate bacterial multiplication (Boch and Bonas, 2010; Schornack et al.,...

Visualization of grapevine root colonization by the Saharan soil isolate Saccharothrix algeriensis NRRL B-24137 using DOPE-FISH microscopy

01/09/2013 00:00
Stéphane Compant, Saima Muzammil, Ahmed Lebrihi and Florence Mathieu   Résumé: There is currently a gap of knowledge regarding whether some beneficial bacteria isolated from desert soils can colonize epi- and endophytically plants of temperate regions. In this study, the early steps of the colonization process of one of these bacteria, Saccharothrix algeriensis NRRL B-24137, was studied on grapevine roots to determine if this beneficial strain can colonize a non-natural host plant. An...

A one-pot enzymatic approach to the O-fluoroglucoside of N-methylanthranilate

15/08/2013 00:00
Lorenzo Caputi, Martin Rejzek, Thomas Louveau, Ellis C. O’Neill, Lionel Hill, Anne Osbourn and Robert A. Field   Résumé: In connection with prospective 18F-PET imaging studies, the potential for enzymatic synthesis of fluorine-labelled glycosides of small molecules was investigated. Approaches to the enzymatic synthesis of anomeric phosphates of d-gluco-configured fluorosugars proved ineffective. In contrast, starting in the d-galacto series and relying on the consecutive action of...

Control of NO level in rhizobium-legume root nodules: Not only a plant globin story

14/08/2013 00:00
Eliane Meilhoc, Pauline Blanquet, Yvan Cam and Claude Bruand   Résumé: Nitric oxide (NO) is a gaseous signaling molecule which plays both regulatory and defense roles in animals and plants. In the symbiosis between legumes and rhizobia, NO has been shown to be involved in bacterial infection and nodule development steps as well as in mature nodule functioning. We recently showed that an increase in NO level inside Medicago truncatula root nodules also could trigger premature nodule...

Use of beneficial bacteria and their secondary metabolites to control grapevine pathogen diseases

01/08/2013 00:00
Stéphane Compant, Günter Brader, Saima Muzammil, Angela Sessitsch, Ahmed Lebrihi, Florence Mathieu   Résumé: Grapevine is one of the most important economic crops yielding berries, wine products as well as derivates. However, due to the large array of pathogens inducing diseases on this plant, considerable amounts of pesticides—with possible negative impact on the environment and health—have been used and are currently used in viticulture. To avoid negative impacts of such products and to...

A conserved rubredoxin is necessary for photosystem II accumulation in diverse oxygenic photoautotrophs

30/07/2013 00:00
Calderon RH, García-Cerdán JG, Malnoë A, Cook R, Russell JJ, Gaw C, Dent RM, de Vitry C, Niyogi KK.   Résumé: In oxygenic photosynthesis, two photosystems work in tandem to harvest light energy and generate NADPH and ATP. Photosystem II (PSII), the protein-pigment complex that uses light energy to catalyze the splitting of water, is assembled from its component parts in a tightly regulated process that requires a number of assembly factors. The 2pac mutant of the unicellular green alga...

Pathogen-associated molecular pattern-triggered immunity and resistance to the root pathogen Phytophthora parasitica in Arabidopsis

12/07/2013 00:00
Mathieu Larroque, Elodie Belmas, Thomas Martinez, Sophie Vergnes, Nathalie Ladouce, Claude Lafitte, Elodie Gaulin and Bernard Dumas   Résumé: The cellulose binding elicitor lectin (CBEL) of the genus Phytophthora induces necrosis and immune responses in several plant species, including Arabidopsis thaliana. However, the role of CBEL-induced responses in the outcome of the interaction is still unclear. This study shows that some of CBEL-induced defence responses, but not necrosis, required...

Post mortem function of AtMC9 in xylem vessel elements

08/07/2013 00:00
Benjamin Bollhöner, Bo Zhang, Simon Stael, Nicolas Denancé, Kirk Overmyer, Deborah Goffner, Frank Van Breusegem, Hannele Tuominen   Résumé: Cell death of xylem elements is manifested by rupture of the tonoplast and subsequent autolysis of the cellular contents. Metacaspases have been implicated in various forms of plant cell death but regulation and execution of xylem cell death by metacaspases remains unknown. Analysis of the type II metacaspase gene family in Arabidopsis thaliana...
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