Optimization of a DNA Nicking Assay to Evaluate Oenocarpus bataua and Camellia sinensis Antioxidant Capacity
09/10/2014 00:00
Louis-Jérôme Leba, Christel Brunschwig, Mona Saout, Karine Martial, Emmanuelle Vulcain, Didier Bereau and Jean-Charles Robinson
Résumé:
This study was aimed at assessing the DNA damage protective activity of different types of extracts (aqueous, methanolic and acetonic) using an in vitro DNA nicking assay. Several parameters were optimized using the pUC18 plasmid, especially FeSO4, EDTA, solvent concentrations and incubation time. Special attention has been paid to removing the...