Liste des publications scientifiques

Characterization of a cytochrome P450 monooxygenase gene involved in the biosynthesis of geosmin in Penicillium expansum

23/05/2012 00:00
Muhammad Hussnain Siddique, Thierry Liboz, Nafees Bacha, Olivier Puel, Florence Mathieu and Ahmed Lebrihi   Résumé: Geosmin  is  a  terpenoid,  an  earthy-smelling  substance  associated  with  off-flavors  in  water  and  wine. The biosynthesis of geosmin is well characterized in bacteria, but little is known about its production in eukaryotes, especially in filamentous fungi. The origin of geosmin in grapevine is largely...

DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage

18/05/2012 00:00
Davis EJ, Lachaud C, Appleton P, Macartney TJ, Näthke I, Rouse J   Résumé: Ubiquitin-binding domains (UBDs) are crucial for recruiting many proteins to sites of DNA damage. Here we characterize C1orf124 (Spartan; referred to as DVC1), which has an UBZ4-type UBD found predominantly in DNA repair proteins. DVC1 associates with DNA replication factories and localizes to sites of DNA damage in human cells, in a manner that requires the ability of the DVC1 UBZ domain to bind to ubiquitin...

Fate of polyphenols and antioxidant activity of barley throughout malting and brewing

01/05/2012 00:00
Céline Leitao, Eric Marchioni, Martine Bergaentzlé, Minjie Zhao, Luc Didierjean, Laurence Miesch, Emilie Holder, Michel Miesch and Saïd Ennahar   Résumé: Phenolic contents of barley and malt extracts and their corresponding antioxidant activities were investigated using a chromatographic online antioxidant detection system. Ethyl acetate extracts of barley and malt were separated using reverse phase HPLC and compounds eluting from the column were submitted to two UV–visible detections: one...

Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling

20/03/2012 00:00
Dong Xin Feng, Céline Tasset, Mathieu Hanemian, Xavier Barlet, Jian Hu, Dominique Trémousaygue, Laurent Deslandes and Yves Marco   Résumé: Means to control bacterial wilt caused by the phytopathogenic root bacteria Ralstonia solanacearum are limited. Mutants in a large cluster of genes (hrp) involved in the pathogenicity of R. solanacearum were successfully used in a previous study as endophytic biocontrol agents in challenge inoculation experiments on tomato. However, the molecular...

Genome-Wide Identification, Functional Analysis and Expression Profiling of the Aux/IAA Gene Family in Tomato

24/02/2012 00:00
Corinne Audran-Delalande, Carole Bassa, Isabelle Mila, Farid Regad, Mohamed Zouine and Mondher Bouzayen   Résumé: Auxin is a central hormone that exerts pleiotropic effects on plant growth including the development of roots, shoots, flowers and fruit. The perception and signaling of the plant hormone auxin rely on the cooperative action of several components, among which auxin/indole-3-acetic acid (Aux/IAA) proteins play a pivotal role. In this study, we identified and comprehensively...

Molecular and biochemical aspects of plant terrestrialization

20/02/2012 00:00
Pierre-Marc Delaux, Amrit Kaur Nanda, Catherine Mathé, Nathalie Sejalon-Delmas and Christophe Dunand   Résumé: The transition from water to land was a major evolutionary step for the green lineage. Based on fossil data, this event probably occurred some 480–430 million years ago, during the Ordovician and the early Silurian and initiated the explosive evolution that led to the modern diversity of photosynthetic organisms living on Earth. The chronological steps are still puzzling, but the...

Identification of the protein sequence of the type III effector XopD from the B100 strain of Xanthomonas campestris pv campestris.

01/02/2012 00:00
Joanne Canonne, Carole Pichereaux, Daniel Marino, Dominique Roby, Michel Rossignol and Susana Rivas   Résumé: During evolution, pathogens have developed sophisticated strategies to suppress plant defense responses and promote successful colonization of their hosts. In their attempt to quell host resistance, Gram-negative phytopathogenic bacteria inject type III effectors (T3Es) into plant cells, where they typically target plant components essential for the establishment of defense...

Bacterial effectors target the plant cell nucleus to subvert host transcription.

01/02/2012 00:00
Joanne Canonne and Susana Rivas   Résumé: In order to promote virulence, Gram-negative bacteria have evolved the ability to inject so-called type III effector proteins into host cells. The plant cell nucleus appears to be a subcellular compartment repeatedly targeted by bacterial effectors. In agreement with this observation, mounting evidence suggests that manipulation of host transcription is a major strategy developed by bacteria to counteract plant defense responses. It has been...

Arabidopsis EDS1 connects pathogen effector recognition to cell compartment-specific immune responses

09/12/2011 00:00
Heidrich K, Wirthmueller L, Tasset C, Pouzet C, Deslandes L, Parker JE   Résumé: Pathogen effectors are intercepted by plant intracellular nucleotide binding–leucine-rich repeat (NB-LRR) receptors. However, processes linking receptor activation to downstream defenses remain obscure. Nucleo-cytoplasmic basal resistance regulator EDS1 (ENHANCED DISEASE SUSCEPTIBILITY1) is indispensible for immunity mediated by TIR (Toll–interleukin-1 receptor)–NB-LRR receptors. We show that Arabidopsis EDS1...

The Arabidopsis thaliana DNA-Binding Protein AHL19 Mediates Verticillium Wilt Resistance

01/12/2011 00:00
Koste A. Yadeta, Mathieu Hanemian, Patrick Smit, Jelle A. Hiemstra, Andy Pereira, Yves Marco and Bart P. H. J. Thomma   Résumé: Verticillium spp. are destructive soilborne fungal pathogens that cause vascular wilt diseases in a wide range of plant species. Verticillium wilts are particularly notorious, and genetic resistance in crop plants is the most favorable means of disease control. In a gain-of-function screen using an activation-tagged Arabidopsis mutant collection, we identified...
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