Liste des publications scientifiques

Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9

19/04/2015 00:00
Alejandro Rojas-Fernandez, Lina Herhaus, Thomas Macartney, Christophe Lachaud, Ronald T. Hay & Gopal P. Sapkota   Résumé: CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high expression and sophisticated tools such as confocal microscopy and flow cytometry. Therefore, a simple, sensitive...

Identification of a stem rust resistance locus effective against Ug99 on wheat chromosome 7AL using a RAD-Seq approach

16/04/2015 14:26
Vincent Pujol, Kerrie L. Forrest, Peng Zhang, Matthew N. Rouse, Matthew J. Hayden, Li Huang, Linda Tabe, Evans Lagudah   Résumé: Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is responsible for major production losses around the world. The development of resistant cultivars is an effective and environmentally friendly way to manage the disease, but outbreaks can occur when new pathogen races overcome the existing host resistance genes. Ug99 (race TTKSK) and related Pgt...

The ancestral role of nodal signalling in breaking L/R symmetry in the vertebrate forebrain

30/03/2015 00:00
Ronan Lagadec, Laurent Laguerre, Arnaud Menuet, Anis Amara, Claire Rocancourt, Pierre Péricard, Benoît G. Godard, Maria Celina Rodicio, Isabel Rodriguez-Moldes, Hélène Mayeur, Quentin Rougemont, Sylvie Mazan & Agnès Boutet   Résumé: Left-right asymmetries in the epithalamic region of the brain are widespread across vertebrates, but their magnitude and laterality varies among species. Whether these differences reflect independent origins of forebrain asymmetries or taxa-specific...

Large-scale insertional mutagenesis of Chlamydomonas supports phylogenomic functional prediction of photosynthetic genes and analysis of classical acetate-requiring mutants

25/02/2015 00:00
Rachel M. Dent, Marina N. Sharifi, Alizée Malnoë, Cat Haglund, Robert H. Calderon, Setsuko Wakao and Krishna K. Niyogi   Résumé: Chlamydomonas reinhardtii is a unicellular green alga that is a key model organism in the study of photosynthesis and oxidative stress. Here we describe the large-scale generation of a population of insertional mutants that have been screened for phenotypes related to photosynthesis and the isolation of 459 flanking sequence tags from 439 mutants. Recent...

Emergence of terpene cyclization in Artemisia annua

03/02/2015 00:00
Melissa Salmon, Caroline Laurendon, Maria Vardakou, Jitender Cheema, Marianne Defernez, Sol Green, Juan A. Faraldos & Paul E. O’Maille   Résumé: The emergence of terpene cyclization was critical to the evolutionary expansion of chemical diversity yet remains unexplored. Here we report the first discovery of an epistatic network of residues that controls the onset of terpene cyclization in Artemisia annua. We begin with ​amorpha-4,11-diene synthase (​ADS) and ​(E)-β-farnesene synthase...

An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat

14/01/2015 00:00
Ke Wang, Shahidul Islam, Junhong Ma, Masood Anwar, Jing Chen, Yueming Yan, Rudi Appels and Wujun Ma   Résumé: Wheat bread-making quality is mainly determined by glutenin proteins in the grain, which exist in a wide range of variable alleles with differential influence on processing attributes. A recently identified allele, Bx7 over-expression (Bx7oe), has been showing highly significant positive effects on wheat dough strength over the normally expressed Bx7 allele. SDS-PAGE and normal...

The N-glycan Cluster from Xanthomonas campestris pv. campestris: a Toolbox for Sequential Plant N-glycan Processing

13/01/2015 00:00
Stéphanie Dupoiron, Claudine Zischek, Laetitia Ligat, Julien Carbonne, Alice Boulanger, Thomas Dugé de Bernonville, Martine Lautier, Pauline Rival, Matthieu Arlat, Elisabeth Jamet, Emmanuelle Lauber and Cécile Albenne   Résumé: N-glycans are widely distributed in living organisms but represent only a small fraction of carbohydrates found in plants. This probably explains why they have not previously been considered as substrates exploited by phytopathogenic bacteria during plant infection....

The CRE1 cytokinin pathway is differentially recruited depending on Medicago truncatula root environments and negatively regulates resistance to a pathogen

06/01/2015 00:00
Carole Laffont, Thomas Rey, Olivier André, Mara Novero, Théophile Kazmierczak, Frédéric Debellé, Paola Bonfante, Christophe Jacquet, Florian Frugier   Résumé: Cytokinins are phytohormones that regulate many developmental and environmental responses. The Medicago truncatula cytokinin receptor MtCRE1 (Cytokinin Response 1) is required for the nitrogen-fixing symbiosis with rhizobia. As several cytokinin signaling genes are modulated in roots depending on different biotic and abiotic...

USP45 deubiquitylase controls ERCC1–XPF endonuclease-mediated DNA damage responses

23/12/2014 00:00
Ana B Perez-Oliva, Christophe Lachaud, Piotr Szyniarowski, Ivan Muñoz, Thomas Macartney, Ian Hickson, John Rouse, Dario R Alessi   Résumé: Reversible protein ubiquitylation plays important roles in various processes including DNA repair. Here, we identify the deubiquitylase USP45 as a critical DNA repair regulator. USP45 associates with ERCC1, a subunit of the DNA repair endonuclease XPF–ERCC1, via a short acidic motif outside of the USP45 catalytic domain. Wild-type USP45, but not a USP45...

Workflow4Metabolomics: A collaborative research infrastructure for computational metabolomics

19/12/2014 00:00
Franck Giacomoni, Gildas Le Corguillé, Misharl Monsoor, Marion Landi, Pierre Pericard, Mélanie Pétéra, Christophe Duperier, Marie Tremblay-Franco, Jean-François Martin, Daniel Jacob, Sophie Goulitquer, Etienne A. Thévenot, Christophe Caron   Résumé: The complex, rapidly-evolving field of computational metabolomics calls for collaborative infrastructures where the large volume of new algorithms for data preprocessing, statistical analysis and annotation can be readily integrated whatever...
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